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The role of the sRNA cosR in the oxidative stress response of Caulobacter crescentus (750.2)
Author(s) -
Ramos Kevin,
Ngadiaga Djamila,
Laub Michael,
Peterson Celeste
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.750.2
Subject(s) - caulobacter crescentus , oxidative stress , catalase , reactive oxygen species , transfer rna , microbiology and biotechnology , biology , oxidative phosphorylation , biochemistry , chemistry , gene , rna , bacterial protein
The ability to survive oxidative stress is crucial for all aerobically growing organisms. Reactive oxygen species (ROS) can disrupt cell membranes, proteins, electron transport chain, and DNA if they are not mitigated. Here we examine the role of a sRNA in the oxidative stress response in the model alpha‐proteobacterium Caulobacter cresentus. Upon oxidative stress, C. crescentus activates the transcription factor OxyR, which in turn directly regulates catalase, alphadyroxyl peroxidase and a sRNA called cosR. We monitor the survival of C. crescentus after exposure to hydrogen peroxide using colony forming unit viability assays and live dead stains. We find that overexpression of the sRNA confers protection to oxidative stress while the deletion of the sRNA causes mild impairment in survival after oxidative stress. Thus, OxyR appears to both directly and indirectly, through the sRNA cosR, activate genes that help the C. crescentus survive oxidative stress.