Premium
Inhibiting HIV‐1 with chimeric tRNA‐miRNA mimics (749.8)
Author(s) -
Gao Eva,
Haushalter Karl
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.749.8
Subject(s) - microrna , transfer rna , rna interference , small hairpin rna , luciferase , rna , biology , computational biology , rna polymerase iii , gene , microbiology and biotechnology , genetics , transfection , rna dependent rna polymerase
Gene therapy utilizing RNA interference (RNAi) has the potential to repress HIV‐1 replication in patients living with HIV. This study investigates the effectiveness of microRNA (miRNA) mimics fused to tRNA in inhibiting the synthesis of proteins important to HIV‐1’s lifecycle. Expression vectors coding for chimeric RNA molecules consisting of tRNA(Arg) fused to miRNA mimics directed against either Gag‐Pol or CCR5 were designed and constructed. Using a dual‐luciferase assay in cultured cells, the effectiveness of these tRNA‐miRNA mimic constructs will be compared with the effectiveness of analogous tRNA‐short hairpin (shRNA) constructs. Furthermore, the miRNA mimic constructs will be tested to determine whether they interfere with endogenous miRNA.