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Cellular and molecular characteristics of neonatal skin: consequences for healing (734.10)
Author(s) -
Krejci Eliska,
Dvorankova Barbora,
Kvasilova Alena,
Borsky Jiri,
Grim Milos,
Smetana Karel
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.734.10
Subject(s) - sox2 , wound healing , homeobox protein nanog , skin repair , mesenchymal stem cell , stem cell , population , medicine , vimentin , pathology , microbiology and biotechnology , biology , embryonic stem cell , induced pluripotent stem cell , surgery , immunohistochemistry , genetics , environmental health , gene
Wound healing is a complex and dynamic process of restoring tissue integrity and layers of skin. While in adults healing results in replacement of normal skin structures with scar tissue, the process is scarless during initial periods of prenatal development. Reduced scar formation was also observed shortly after birth. Having the opportunity to obtain newborn skin after corrective surgery of cleft lip, we aimed to compare properties of cellular elements from neonatal and older skin that could be responsible for nearly scarless healing. Cultivated neonatal fibroblasts expressed Nestin, a marker of immature cells, and markers of stem cells Nanog, OCT3/4, SOX2. Neonatal fibroblasts also retained some degree of plasticity as they could be differentiated into adipocytes, smooth muscle cells and chondroblasts but not to osteoblasts. Cultivated neonatal keratinocytes included population of very small cells (4 µm in diameter) exhibiting both epithelial (keratin) and mesenchymal (vimentin) marker, as well as some epidermal stem cell markers. These preliminary data suggest that minimal scarring of neonatal skin could be at least partially explained by persistence of some prenatal properties of cells that retained stem cell characteristics. Grant Funding Source : Supported by GACR project nr. 13‐20293S, PRVOUK project nr. P27/LF1/1 and UNCE project nr. 204013.