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Encapsulation of biological stains for drug delivery imaging and microscopy in dentin tubules (733.6)
Author(s) -
Lau Michael,
Haseeb Ridwan,
Montagner Francisco,
Rodrigues Danieli
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.733.6
Subject(s) - dentin , penetration (warfare) , drug delivery , stain , emulsion , particle size , fluorescence microscope , materials science , chemistry , chromatography , chemical engineering , biomedical engineering , nanotechnology , staining , fluorescence , composite material , pathology , biochemistry , medicine , physics , operations research , quantum mechanics , engineering
Encapsulation of Biological Stains for Drug Delivery Imaging and Microscopy in Dentin Tubules Michael Lau 1 , Ridwan Haseeb 1 , Francisco Montagner 2 , Danieli Rodrigues 1 : 1 Department of Bioengineering, University of Texas at Dallas, Richardson, TX; 2 Department of Conservative Dentistry, Federal University of Rio Grande do Sul, Brazil; Introduction: Bacteria are able to remain into dentin after the root canal disinfection. Drug penetration into the tubules can be a key component for infection control. This study uses the synthesis of poly(ethylene glycol)‐block‐poly(L‐lactide) PEG/PLA encapsulated biological stain particles to characterize the penetration depth of drug delivery systems with Confocal Laser Scanning Microscopy (CLSM). Methodology : Encapsulation of the biological stain was done with an oil‐water emulsion‐evaporation technique. The PEG/PLA copolymer and the stain were dissolved in the oil phase while the surfactant was dissolved in water. The oil phase and water phase was combined and emulsified then stirred. The particles were centrifuged, washed, and lyophilized. Characterizing the particle size and fluorescence was done with CLSM (VK‐X200 Keyence Laser Scanning Microscope). Results: The microscopy has shown particle formation in the 0.3‐1.0 m range, which depended on the method of emulsification: homogenizing or sonication. CLSM revealed that the particles fluoresce under light with 525‐605 nm wavelengths. Conclusion: The encapsulation technique effectively encapsulated the biological stain to penetrate the dentin tubules. With particle size similar to dentin morphology, the encapsulated stain proliferation into the dentin tubules will model dentin penetration of drug delivery systems of similar size.