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De novo identification of non‐coding RNAs in aging male germline stem cells (732.8)
Author(s) -
Luk Alfred,
Ng Shuk Han,
Lee TinLap
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.732.8
Subject(s) - germline , biology , stem cell , long non coding rna , non coding rna , phenotype , rna , genetics , computational biology , gene
Aging is a complex time‐dependent biological process that takes place in every cell and organ, eventually leading to degenerative changes that affect normal biological functions. In the past decades, the number of older parents has increased significantly. While it is widely recognized that advanced maternal age poses higher birth defect risk due to egg aging, the effect of advanced paternal aging remains largely overlooked. Non‐coding RNAs are generally classified as non‐ protein‐coding RNAs have emerged as a novel player in many developmental processes. However its role in advanced paternal aging are unknown. Here we show a de novo approach to annotated unique lncRNA transcripts associated with male germline stem cell aging. Based on previous mice aging model consisting of 6‐day, 21‐day, 60‐day and 8‐month old animals. We identified a set of age‐dependent lncRNA transcripts that have not been annotated previously. Preliminary analysis on RNA structure and protein binding suggested the transcripts involved in a wide variety of developmental functions, including proliferation, adhesion and cell death. The findings are expected to substantially change the concepts of lncRNA function in developmental, reproductive and stem cell biology.