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Comparative study of in vitro expanded somatic stem cells from different sources (732.3)
Author(s) -
Danisovic Lubos,
Bohac Martin,
Novakova Zuzana,
Varga Ivan,
Bohmer Daniel,
Polak Stefan
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.732.3
Subject(s) - mesenchymal stem cell , cd90 , chondrogenesis , adipose tissue , microbiology and biotechnology , cd44 , cd34 , dermis , bone marrow , biology , flow cytometry , stem cell , chemistry , in vitro , immunology , anatomy , biochemistry , endocrinology
Mesenchymal stem cells (MSCs) represent a unique tool of regenerative medicine. They are undifferentiated cells with the ability of long‐term self‐renewing and plasticity. The main goal of this study was biological and morphological analysis of human MSCs derived from bone marrow, adipose tissue and dermis in respect to cartilage tissue engineering. MSCs were isolated and cultured in vitro using standard protocols. They were maintained in a‐MEM supplemented by 15% of fetal bovine serum and antibiotics. MSCs from all tissues were cultured up to the 5th passage. The kinetics of proliferation was analyzed by CEDEX XS and expression of selected markers was assessed by flow cytometry. They were morphologically analyzed by inverted microscope and TEM. Pellet cultures were used to induce chondrogenic differentiation and this event was proved by real‐time PCR. MSCs from all sources were attached on the Petri dishes after 24 h and started to proliferate in colonies. After 7‐10 days they reached 80% confluency and were passaged. In the next 5 passages they displayed fibroblastoid morphology. MSCs also showed similar kinetics of proliferation and shared the expression of CD29, CD44, CD73, CD90, CD105 and CD166 and were negative for CD14, CD34, CD45 and HLA‐DR. TEM showed similar morphology of all analyzed MSCs. We have demonstrated that MSCs from bone marrow and adipose had potential to undergo chondrogenic differentiation comparable with chondrocytes, but this potential was decreased in dermis‐derived MSCs. We can emphasize that MSCs isolated from bone marrow, adipose tissue and dermis possess similar biological and morphological properties, but differ in chondrogenic potential. Dermis‐derived MSCs have an inferior potential for chondrogenic differentiation. So this fact decreases their potential for cartilage tissue engineering. Hoverer, further studies focused on applying of appropriate exogenous factors must be carried out to clarify this issue. Grant Funding Source : Supported by MZSR no. 2012/4‐UKBA‐4 and APVV no. 0434‐12.