Premium
In vitro skeletal myofiber disassembly and recovery with cardiotoxin (731.9)
Author(s) -
Mattioli Patrisia,
Hernanadez Elena,
Bratton Stephaie,
Bova Michael,
DiLullo Camille
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.731.9
Subject(s) - cardiotoxin , myocyte , myogenesis , skeletal muscle , biology , in vivo , andrology , toxin , in vitro , medicine , anatomy , endocrinology , biochemistry , genetics
Injection of cobra venom cardiotoxin into skeletal and cardiac muscle In Vivo causes injury to the respective muscle myofibers. This cardiotoxin has been shown specifically to disassemble myofibers in the muscle while leaving the surrounding tissues unaffected. To determine if cardiotoxin would similarly affect skeletal muscle In Vitro, primary embryonic chick skeletal muscle cultures were exposed to low doses of cardiotoxin from Naja mossambica mossambica. Cells at 4 days in culture were exposed to toxin for 48 hours. Toxin was washed out after the treatment period. Cultures were fed daily, fixed on days 1‐3 after wash out and immunofluorescently labeled for muscle specific proteins. Immediately after wash‐out it was observed that myofibers had disassembled in the majority of myocytes. Preliminary data indicated that nuclear counts between treated and untreated cultures remained relatively constant suggesting limited cell death in treated cultures. Compared to control cultures, length and girth of treated myocytes as well as number of nuclei per myotube were negatively impacted by toxin exposure. After wash‐out toxin treated cultures exhibited thin, unstriated myofibers in mononucleated myocytes by day 2 of recovery. By day 3 of recovery myotubes with 2‐3 nuclei, slightly increased girth and small numbers of myofibers with alpha‐actinin striations were detected.