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Menstrual cycle change in thalamic glutamate decarboxylase and GABA in response to TMJ pain in rats (726.4)
Author(s) -
Umorin Mikhail,
Bellinger Larry,
Kramer Phillip
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.726.4
Subject(s) - glutamate decarboxylase , estrogen , endocrinology , medicine , menstrual cycle , thalamus , luteinizing hormone , gabaergic , glutamate receptor , hormone , chemistry , biology , enzyme , neuroscience , biochemistry , receptor , inhibitory postsynaptic potential
Women report TMJ pain more often than men and epidemiological evidence suggests the pain response varies between men and women and over the menstrual cycle. Recent studies show TMJ responsive neurons projecting to the thalamus have different activities in male and females. Using a rat model our lab performed whole genome array screens on thalamic tissue of cycling rats. The results indicate that the transcript of both glutamate decarboxylase 1 and 2 (GAD67 and GAD65, respectively) are significantly up‐regulated in the thalamus of proestrus (high estrogen, progesterone and luteinizing hormone concentration) versus diestrus/metestrus (low estrogen, progesterone and luteinizing hormone concentration) rats. On the other hand, GABA expression (by ELISA assay) did not significantly change in the thalamic tissue during the proestrus phase. The differences in response of the enzyme and its product could be explained by the distribution of each enzyme within individual neurons. In conclusion, sex steroids could alter thalamic GABAergic signaling to effect TMJ hyperalgesia through regulation of GAD. Grant Funding Source : Supported by NIH R01 NIDCR grant: DE022129 Estrogen and TMJ Pain