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The low‐level laser and natural latex protein applied on the sciatic nerve crushed: morphometric and scanning electron microscopy study (726.11)
Author(s) -
Dias Fernando,
Issa Joao Paulo,
CoutinhoNetto Joaquim,
Fazan Valeria,
Iyomasa Mamie,
Watanabe Iisei
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.726.11
Subject(s) - sciatic nerve , scanning electron microscope , myelin , chemistry , anatomy , materials science , nuclear medicine , medicine , endocrinology , composite material , central nervous system
This study evaluated the effects of low‐level laser (LLL 15J/cm2, 780nm) and F1 protein (natural latex ‐ 0,1%) on the sciatic nerve crushed (15 kgf, axonotmesis). We used 72 rats (♂, 200‐250g) divided into 6 groups (n = 12): GC ‐ control; EG ‐ exposed nerve; IG ‐ injured nerve; LG ‐ LLL; PG ‐ F1; LPG ‐ LLL and F1. After 4 or 8 weeks, the animals were euthanized, the sciatic nerve was processed for morphometric (nerve fibers minimum diameter, myelin thickness and G‐ratio) and scanning electron microscope (SEM) analyzes. The morphometric data after 4 weeks showed improvements with the applied treatments, PG showed better results. After 8 weeks it was observed greater homogeneity among the groups. The G‐ratio showed no tendency to change (4 or 8 weeks), the values were closer after 8 weeks. The SEM data showed normal features in CG and EG, the IG (4 weeks) revealed a huge loss of nerve fibers and endoneurial collagen synthesis, the LG and PG (4 weeks) showed small clusters of nerve fibers. PG and LPG (8 weeks) revealed larger and more juxtaposed fibers in comparison to the injured groups discussed at 4 weeks. The treatment with the F1 and LLL suggest an improvement in the recovery of crushed peripheral nerve (4 and 8 weeks). After 8 weeks, the treated groups were more similar to each other, revealing the time‐dependency. The groups that received F1 associated or not to LLL showed the better results. Grant Funding Source : FAPESP 2010/12018‐3