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Increased Ca 2+ permeable ampa receptor expression following carotid body denervation in goats (713.2)
Author(s) -
Miller Justin,
Neumueller S,
Muere C,
Olesiak S,
Daghistany A,
Pan L,
Hodges M,
Forster H
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.713.2
Subject(s) - ampa receptor , carotid body , denervation , ventilation (architecture) , nmda receptor , glutamate receptor , respiratory center , respiratory system , receptor , medicine , brainstem , chemistry , endocrinology , hypoventilation , biology , anesthesia , mechanical engineering , stimulation , engineering
The mechanisms which contribute to the time‐dependent recovery of resting ventilation and the ventilatory CO 2 chemoreflex after carotid body denervation (CBD) are poorly understood. The initiation of neuronal plasticity often involves changes in Ca 2+ permeability. Therefore, we tested the hypothesis that during recovery from the acute effects of CBD in goats, there is an increase in Ca 2+ permeable GluA1R and GluN1R and/or a decrease in Ca 2+ impermeable GluA2R expression at brainstem sites that contribute to respiratory control. We tested this hypothesis via optical densitometric (OD) quantification of medullary neurons immunostained with antibodies targeting GluA1R, GluA2R, and GluN1R at 5‐13 days or 30 days after bilateral CBD. CBD resulted in hypoventilation and a reduced CO 2 chemoreflex, which both recovered significantly over 30 days post‐CBD. At 5‐13 days post‐CBD, GluA1 OD was unchanged from control goats, but GluA2 and GluN1 OD were reduced 15‐30% within 6 of medullary respiratory nuclei measured. However, at 30 days post‐CBD GluA1 OD remained unchanged, but GluA2 and GluN1 OD returned to control levels. We conclude that a shift toward Ca 2+ permeable glutamate receptors 5‐13 days post‐CBD may initiate the recovery of ventilation after CBD. Furthermore, at 30 days post‐CBD the partial recovery of ventilation is maintained by relatively normal glutamate receptor expression. Grant Funding Source : Supported by National Heart, Lung, and Blood Institute Grants HL25739 and HL007852 and by VA