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Aerobic interval training increases circulating number and improves function of monocyte‐derived endothelial progenitor cells in sedentary men (706.5)
Author(s) -
Tsai HsingHua,
Chen RongRong,
Wang JongShyan
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.706.5
Subject(s) - cd16 , cd14 , progenitor cell , monocyte , endothelial progenitor cell , medicine , endocrinology , cd34 , flow cytometry , andrology , receptor , immunology , biology , stem cell , immune system , cd3 , microbiology and biotechnology , cd8
This study investigated the effects of aerobic interval (AIT) and moderate continuous training (MCT) on circulating number and function of monocyte‐derived endothelial progenitor cells (EPCs) in sedentary men. Twenty‐four sedentary males were randomized to perform AIT (3‐minute intervals at 40% and 80% VO2 peak, n=12) or MCT (sustained 60% VO2 peak, n=12) for 30 minutes/day, 5 days/week for 6 weeks. Counts of CD34+and kinase domain receptor (KDR)+ monocytes attributed to specic monocyte subsets [CD14++/CD16‐ (Mon1), CD14++/CD16+ (Mon2), and CD14+/CD16++ (Mon3)] by four‐color flow cytometry. Endothelial function was measured by endothelium‐dependent ow‐mediated vasodilatation (FMD). Late out‐growth EPCs were cultured to evaluate wounding migration and tube formation. The experimental results showed that both AIT and MCT increased circulating level of CD34+/KDR+ Mon2 cells. Moreover, FMD was markedly higher with AIT compared to MCT. However, AIT rather than MCT significantly enhanced in vitro wounding migration and tube formation. Hence we conclude that AIT effectively increases circulating number and improves function of monocyte‐derived EPCs in sedentary men.