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microRNA‐206 modulates important regulators of gene expression in response to aerobic exercise training in human skeletal muscle (706.3)
Author(s) -
Alves Cleber,
Junior José,
Fernandes Tiago,
Alves Guilherme,
Negrão Carlos,
Oliveira Edilamar
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.706.3
Subject(s) - myod , myf5 , skeletal muscle , aerobic exercise , medicine , endocrinology , cardiorespiratory fitness , endurance training , myogenesis , myogenin , biology
Aerobic exercise training (AET) has been established as an important phenotype capable of altering the human skeletal muscle. MicroRNAs (miRNAs) are regulators of biological processes by modulating gene expression. The microRNA‐206 is skeletal muscle‐specific. Therefore, we evaluate the effects of expression of miR‐206 in response to AET in human skeletal muscle. Twelve healthy volunteers were biopsied pre and post period aerobic exercise training. Moreover, heart rate (HR), mean blood pressure (MBP), maximal exercise capacity (VO 2peak ) forearm blood flow (FBF) and forearm vascular conductance (FVC) were evaluated, the miRNA‐206, PAX‐7, MYF5, MYOD, MYOG levels were analyzed. Aerobic exercise training was performed for 16 weeks. All variables were re‐assessed following completion of the training period. After physical training, the individuals showed an increase of 97% in human skeletal muscle in microRNA‐206 expression levels. These alterations were accompanied by increased of 55%,84%,65%, 80% in PAX‐7, MYF5, MYOD, MYOG expression level, respectively. However, when analyzed by western blot comparing pre and post period there is decreased of 21% in MYOD and 33% in PAX‐7 protein levels, but MHC and MYOG there were increase of 44% and 40% in protein levels, respectively. Furthermore, decreased of resting bradycardia 12,5%, increased of VO 2peak 18%, increased FBF 68% and increased of FVC 63%. These results suggest that in healthy subjects the miRNA miR‐206 is highly expressed after aerobic exercise training can thus locally modulating some signaling ways in muscle regeneration.