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The role of skeletal myofiber VEGF in the cerebral neurogenic response to exercise (706.21)
Author(s) -
Rich Benjamin,
Scadeng Miriam,
Wagner Peter,
Breen Ellen
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.706.21
Subject(s) - neun , neurogenesis , endocrinology , medicine , myocyte , vascular endothelial growth factor , cerebral blood flow , skeletal muscle , vegf receptors , chemistry , biology , neuroscience , immunohistochemistry
Exercise induces cerebral neurogenesis. This phenomenon has been suggested to require peripheral vascular endothelial growth factor (VEGF). We hypothesized that skeletal myofiber VEGF may regulate blood flow and neurogenesis in the hippocampus. This was tested in adult skeletal myofiber specific VEGF gene ablated mice (skmVEGF‐/‐) provided running wheels for 14 days. We measured exercise capacity, neurogenesis (BrDU+/NeuN+ cells) and post‐training cerebral blood flow (CBF) detected by fMRI. Voluntary distances ran each day did not differ between groups. There was a trend for higher BrDU+/NeuN+ cells/0.1 mm 2 in WT mice (WT, 4.8 + 2.0, skmVEGF‐/‐ 1.8 + 0.6; p=0.08). CBF in mice breathing 21% or 10% O 2 did not differ between groups. Endurance was increased in WT compared to skmVEGF‐/‐ mice post‐training (Pre‐training; WT 80 + 15 min., skmVEGF‐/‐, 27 + 5 min., Post‐training; WT 107 + 21 min., skmVEGF‐/‐, 42 + 8 min., p<0.05). Speed improved in WT mice with training (Pre‐training; WT 61 + 2 cm/s, skmVEGF‐/‐, 51 + 3 cm/s, Post‐training; WT 69 + 3 cm/s, skmVEGF‐/‐, 61 + 4 cm/s, p<0.05). These data suggest that myofiber VEGF, independent of CBF, may regulate exercise‐induced neurogenesis. Grant Funding Source : American Heart Association