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Improvement of body‐fluid dysfunction by Poria cocos in nephrotic syndrome (701.3)
Author(s) -
Lee So Min,
Lee Yun Jung,
Yoon Jung Joo,
Han Byung Hyuk,
Kang Dae Gill,
Lee Ho Sub
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.701.3
Subject(s) - nephrotic syndrome , epithelial sodium channel , nephrosis , medicine , endocrinology , proteinuria , triglyceride , sgk1 , kidney , chemistry , cholesterol , glucocorticoid , sodium , organic chemistry
Improvement of body‐fluid dysfunction by Poria cocos in nephrotic syndrome So Min Lee 1,2 , Yun Jung Lee 1,2 , Jung Joo Yoon 1,2 , Byung Hyuk Han 1,2 , Dae Gill Kang 1,2 , and Ho Sub Lee 1,21 College of Oriental Medicine and Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk 570‐749, Republic of Korea; 2 Hanbang Body‐fluid Research Center, Wonkwang University, Shinyong‐dong, Iksan, Jeonbuk, 570‐749, Republic of Korea Abstract Nephrotic syndrome is associated with altered renal handling of water and sodium along with changes of aquaporins (AQP) and renal epithelial Na channel (ENaC). The dried sclerotia of Poria cocos Wolf (WPC) has been used for the treatment of chronic edema and nephrosis. This study was conducted to evaluate the effects of WPC on puromycin aminonucleoside (PAN)‐induced renal functional derangement and the change of renal AQP2 and ENaC expression. The nephrotic syndrome animal models were constructed by PAN 75 mg/kg injection and then were treated with losartan (30 mg/kg/day) or WPC (200 mg/kg/day) for 7 days. Consequently, WPC group was significantly improved the proteinuria and ascites. In addition, the plasma level of triglyceride, total cholesterol, and low density lipoprotein (LDL)‐cholesterol were significantly decreased in WPC. Besides, WPC group attenuated the PAN‐induced increase in protein, and mRNA levels of AQP2 and ENaC subunit α/β. WPC was significantly suppressed PAN‐induced organic osmolytes regulator such as serum‐ and glucocorticoid‐inducible protein kinase (Sgk1), and sodium‐myo‐inositol cotransporter (SMIT) mRNA expression. Taken together, WPC improve nephrotic syndrome including proteinuria and ascites, through inhibition of AQP2 and ENaC subunit. Thus, WPC is involved in the body‐fluid regulation via inhibition of water and sodium channels against renal disorder such as edema or nephrosis.