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Chronic treatment with antioxidant flavonoids improves the vascular reactivity in mesenteric arteries from obese rats (700.10)
Author(s) -
Priviero Fernanda,
RojasMoscoso Julio,
De Souza Verônica,
Ravos Amanda,
Claudino Mario,
Rocha Thalita,
Carvalho Patrícia
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.700.10
Subject(s) - oxidative stress , antioxidant , bioavailability , medicine , endocrinology , chemistry , quercetin , hesperidin , fructose , mesenteric arteries , pharmacology , artery , food science , biochemistry , alternative medicine , pathology
Impaired vascular relaxation is often observed in obese individuals, which is likely associated to the decreased NO bioavailability and increased oxidative stress. Antioxidant flavonoids are usually proposed as a therapy against the increased oxidative stress. However, glycosylated flavonoid bioavailability is limited because of its low capacity of intestinal absorption. We aimed to evaluate the effects of a chronic treatment with glycosylate hesperidin (gHD) and its enzymatically hydrolyzed compound hesperetin (HT) on the vascular relaxation of obese rats. After the 4th week of life, rats were fed with standard chow or high fat diet (59% of total fat) + fructose (1mg/ml) for 12 weeks. gHD or HT were administrated by gavage (1ml/rat/day) during the last 8 weeks. Next, we measured body weight and epididimal weight and the superior mesenteric artery was removed in order to obtain concentration response curves to acetylcholine. Endothelium‐independent relaxation was evaluated by a single concentration of sodium nitroprusside (SNP). High fat diet + fructose caused an increase in the final body weight and epididimal fat, which was not prevented by the flavonoids (Final body weight: Control: 414 ± 15g, High Fat Diet: 504 ± 17g, gHD: 497 ± 39g, HT: 536 ± 22g; Epididmal fat: Control: 7 ± 1g, High fat diet: 19 ± 2g, gHD: 15 ± 1g, HT: 21 ±2g) Further, obese rats showed decreased vascular relaxation to acetylcholine (pEC50: 7.35 ± 0.35 vs 5.78 ± 0.42, for control and high fat diet, respectively). However, chronic treatment with antioxidant flavonoids prevented this decrease (pEC50: 6.97 ± 0.11 and 7.53 ± 0.05, for gHD and HT, respectively), suggesting that the impaired relaxation is because of the increased pro oxidant agents. Maximal responses to acetylcholine were similar among the groups. No differences were seen in the relaxant response to 1uM of SNP. In conclusion, either gHD or HT prevented the impaired vascular relaxation observed in obesity with no changes in the body weight and epididimal fat accumulation. Grant Funding Source : Financial Support: FAPESP and CNPq