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Bestrophin‐3 expression in mouse glomeruli (694.1)
Author(s) -
Golubinskaya Veronika,
Elvin Johannes,
Ebefors Kerstin,
Gustafsson Helena,
Mallard Carina,
Nyström Jenny,
Nilsson Holger
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.694.1
Subject(s) - podocyte , glomerulus , biology , gene isoform , microbiology and biotechnology , western blot , kidney , blot , messenger rna , immunohistochemistry , intermediate filament protein , alternative splicing , cell , intermediate filament , cytoskeleton , endocrinology , gene , immunology , biochemistry , proteinuria
The bestrophin isoform, bestrophin‐3 (Best3), acts as a calcium‐activated chloride channel in cardiomyocytes and in vascular smooth muscle. It may have cell‐protective functions in other cell types. Best3 may be regulated by cyclic GMP and exists in different splice variants. We have examined expression and localization of Best3 in mouse kidney on the mRNA level using RT‐PCR and on the protein level by immunohistochemistry and Western blotting. Staining for Best3 was obtained in mouse glomeruli, and was also detected in cultured mouse podocytes. Western blot confirmed the presence of Best3 protein in kidney cortex. Best3 did not co‐localize with markers for endothelial cells or podocyte foot processes. However, it co‐localized with the intermediate filament nestin, suggesting localization of Best3 in podocyte primary processes and cell bodies. In kidney cortex, in glomerulus‐enriched fractions and in cultured podocytes the two shortest splice variants, but no full‐length mRNA, were detected. In conclusion, two variants of Best3, lacking some of the predicted transmembrane segments, are expressed in podocytes in mouse kidney, and are likely co‐localized with the intermediate filaments.