Premium
Short distance transportation stress in the adult domestic chicken as modulated by pretreatment with ascorbic acid and α‐tocopherol (693.18)
Author(s) -
Azeez Odunayo,
Oyagbemi Ademola,
Oyewale Johson
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.693.18
Subject(s) - ascorbic acid , glutathione , malondialdehyde , erythrocyte fragility , chemistry , red blood cell , tocopherol , oxidative stress , zoology , antioxidant , vitamin c , biochemistry , food science , medicine , vitamin e , biology , enzyme , hemolysis
The effects of short distance transportation stress and pretreatment with non‐enzymic antioxidants ‐ ascorbic acid and α‐tocopherol on the haematology, erythrocyte osmotic fragility (EOF), Total plasma protein (TP), H2O2 generation, Malondialdehyde (MDA) and glutathione (GSH) levels were investigated using the domestic chicken. Thirty‐two, adult female domestic chickens of the Nera black strain were used. The birds were divided into four groups A‐D of 8 birds each. Groups A and B did not receive any medication. Group C received ascorbic acid (650mg/kg diet) while D, α‐tocopherol (270mg/kg diet) in feed for 2 weeks. Birds in groups B, C and D were transported through a distance of 200km. Blood samples were collected within 30 minutes after the journey for analysis. Haematocrit (PCV), red blood cell counts (RBC), haemoglobin, erythrocyte indices (MCV, MCH and MCHC) and EOF, TP, H2O2, LP and GSH levels were evaluated after transportation. Group B had lower EOF than the control, while those pretreated with Vitamins C and E were higher. Haemoglobin and MCHC values were higher in Group B while other haematological parameters were similar. There were no significant differences in the total protein, H2O2, MDA and GSH levels in Group B compared with the control. Group C however, had higher H2O2 and MDA levels but lower TP and GSH than the control. Whereas, Group D had higher H2O2 and GSH levels but lower protein than the control. This study demonstrated that nucleated erythrocytes of the domestic chicken respond to acute stress by increase in osmotic resistance and membrane stability in hypotonic solution, this was antagonized by pretreatment with Vitamins C and E. Furthermore, short distance transportation may not increase oxidative stress and lipid peroxidation in the chicken. Prolonged use of the antioxidants especially ascorbic acid may however lead to oxidative stress