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Effects of hyperglycemia and cyclic stretching on MMP‐2 and TGF‐β in rat mesangial cells (689.8)
Author(s) -
White Tiffani,
Spires Denisha,
Roman Richard,
Williams Jan
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.689.8
Subject(s) - matrix metalloproteinase , transforming growth factor , extracellular matrix , medicine , downregulation and upregulation , endocrinology , chemistry , extracellular , mesangial cell , tgf beta 1 , transforming growth factor beta , glomerulosclerosis , biochemistry , kidney , proteinuria , gene
Previous studies have demonstrated that matrix metalloproteinase‐2 (MMP‐2) is involved in cleaving and activating several non‐extracellular matrix substrates as well such as transforming growth factor‐beta (TGF‐β) that participate in the accumulation of mesanigal extracellular matrix leading to glomerulosclerosis during diabetes‐induced renal disease. However, the mechanism by which MMP‐2 becomes activated remains unclear. Therefore, the aim of the present study was to compare the effects of hyperglycemia and cyclic stretching (FlexCell System) on the expression of MMP‐2 and TGF‐β in cultured rat mesangial cells (MCs). MCs were seeded on 6‐well flex‐bottom plates and cultured in normal glucose (NG) medium. After 24 hrs, the wells in each plate were equally divided into NG and high glucose (HG). The next day, MCs were exposed to cyclic stretching for 18 hrs. We did not observe any differences in MMP‐2 and TGF‐β expression between static NG‐ and HG‐treated MCs. However, exposure to cyclic stretching significantly increased the expression of MMP‐2 and TGF‐β protein in both NG and HG‐treated MCs in comparison to static‐treated MCs. Moreover, MMP‐2 activity was markedly increased in response to cyclic stretching regardless of whether hyperglycemia was present. These data indicate that the upregulation of MMP‐2 and TGF‐β in MCs is primarily due to cyclic stretching rather than hyperglycemia.