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Effect of vitamin D3 supplementation on thyroid hormones and deiodinase 2 expression in streptozotocin‐induced diabetic rats (688.9)
Author(s) -
Alrefaie Zienab
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.688.9
Subject(s) - medicine , endocrinology , streptozotocin , diabetes mellitus , thyroid , intraperitoneal injection , vitamin , deiodinase , hormone , iodothyronine deiodinase , triiodothyronine
Aim : The present study aimed to assess the effect of vitamin D3 administration on deiodinase 2 (D2) mRNA expression in various tissues of diabetic rats. Methods: Thirty male Wistar rats (200‐250 g) were included into; control, diabetic and diabetic supplemented with vitamin D3 groups. Diabetes was induced by single intraperitoneal injection of streptozotocin 45 mg/kg. Vitamin D3 was administered orally in a dose of 500 IU/kg/day in olive oil for 10 weeks. Serum levels of free T4, free T3 and TSH were measured. Tissue homogenates from liver, kidney, muscle, femur bone, heart and brain were obtained and assessed for expression of D2 mRNA. Results: Diabetic rats demonstrated significant increase in free T4 and significant decrease in free T3 with non significant increase in TSH compared to control rats. The changes in thyroid profile were ameliorated by vitamin D3 administration to diabetic rats. D2 mRNA expression was significantly reduced in all tissue homogenates of diabetic rats, however vitamin D3 did enhance D2 mRNA expression especially in liver and brain of diabetic rats. Conclusion: Diabetes mellitus inhibited peripheral conversion of T4 into T3 secondary to reduction in the expression of D2 in almost all body tissues. Vitamin D3 greatly corrected the alterations in thyroid profile and D2 expression in diabetic rats and protected against the subclinical hypothyroid state that could associate diabetes mellitus. Grant Funding Source : No