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Cardiac proteome analysis of sex and estrogen receptor β effects in pressure overload (680.4)
Author(s) -
Kararigas Georgios,
Fliegner Daniela,
Forler Stefanie,
Klein Oliver,
Schubert Carola,
Klose Joachim,
RegitzZagrosek Vera
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.680.4
Subject(s) - pressure overload , estrogen receptor , heat shock protein , medicine , endocrinology , proteome , receptor , estrogen , knockout mouse , protein subunit , chemistry , heart failure , biochemistry , cardiac hypertrophy , cancer , breast cancer , gene
The cardiac response to pressure overload (PO) differs between the sexes and estrogen receptor (ER) β is considered cardioprotective. However, the underlying mechanisms are poorly understood. We aimed at the analysis of the effects of sex and ERβ in PO taking a proteomic approach. Two‐month‐old C57BL6 male (M) and female (F) wild‐type (WT) and ERβ knockout (BERKO) mice were subjected to transverse aortic constriction (TAC) or sham surgery ( n = 4/group). The proteome of left ventricular samples was separated by high‐resolution 2‐dimensional polyacrylamide gel electrophoresis. At nine weeks post operation, TAC led to a greater increase in heart‐weight‐to‐tibia‐length ratio in WT M (128%; P < 0.05) than in WT F (54%; P < 0.05) mice, while this difference in BERKO mice between M (53%; P < 0.05) and F (55%; P < 0.05) was abolished. Our comparative proteomic analysis revealed that in WT M and F mice 82 and 31 protein spots, respectively, differed between sham and TAC ( P 蠄 0.05). In BERKO M and F mice we found 114 and 87 altered protein spots, respectively ( P 蠄 0.05). Among others, trifunctional enzyme subunit family members were repressed in M‐WT‐TAC mice, while cytoskeletal proteins were induced in F‐WT‐TAC mice. On the other hand, heat shock proteins were induced in both M and F‐BERKO‐TAC mice. In conclusion, our study demonstrates that both sex and ERβ influence the proteomic response of the heart to PO. Grant Funding Source : German Research Foundation (DFG, FG 105, GK 754)