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Functional changes in cardiovascular responses with exposure to oxidative insult in a zebrafish embryo model (674.12)
Author(s) -
Frame Mary,
Gunter Geoffrey,
Thompson Holly
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.674.12
Subject(s) - aorta , oxidative stress , medicine , acetylcholine , chemistry , constriction , endocrinology , phenylephrine , zebrafish , heart rate , anatomy , anesthesia , biochemistry , blood pressure , gene
Our goals were to design an observation chamber to observe the cardiovascular responses of anesthetized (0.1% MS222) zebrafish embryos (N=50) at 4‐5 dpf (days post fertilization), and to determine if oxidative insult altered these responses. The chamber was molded out of a silicone elastomer, and consisted of a shallow depression to hold the fish, surrounded by a larger moat that is maintained at 28.5C. Without restraining the fish, this system permits volume exchange (to test different doses/agents) while keeping the fish continuously within the field of view. Heart rate (HR), and diameter (D) changes for the aorta and caudal artery were obtained in response to acetylcholine (ACH 1e‐4M), nitroprusside (NP 1e‐4M), adenosine (ADO 1e‐4M) and phenylephrine (PE 1e‐6M). All solutions were made in instant ocean without methylene blue. The baseline D were ~50 um (aorta) and ~30 um (caudal a.). Baseline HR was 96 b/m. Maximal dilation values were 25% ACH, 30% NP, 20% ADO; maximal constriction to PE was ‐50%. Changes in HR were ‐74% ACH, ‐40% NP, ‐50% ADO, and +20% PE. Oxidative stress was induced by exposure to 1e‐4M peroxide, or 0.25 ug/ml nanometal oxide (silica, ceria, alumina) for 24 hours at 28.5C. Following oxidative stress, dilation and decreased HR to ACH was attenuated, consistent with endothelial dysfunction. Responses to NP or ADO were unchanged. Responses to PE were an accentuated constriction, but unchanged HR. This chamber provides a means to continuously observe vasoactive responses within the zebrafish embryo. The zebrafish model has the potential to test for oxidative insult impact on the cardiovascular system.