Premium
Fullerene C60 chemically linked for biocompatible polymers for in vivo radical trapping (663.2)
Author(s) -
Tegegn Cheregn,
Megos Welay,
Simpson Duane,
Volkis Victoria
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.663.2
Subject(s) - radical , chemistry , fullerene , polymer , biocompatible material , photochemistry , anthracene , combinatorial chemistry , organic chemistry , medicine , biomedical engineering
Free radicals can be formed in living tissues under UV irradiation and/or when oxygen interacts with certain bio‐molecules. Once formed these highly reactive particles are capable to start number of chain reactions damaging living tissues and are believed to be a reason for some kinds of cancer. It is thus important to capture and study free radicals in live biological tissues and samples, which is complicated due to the short‐lived life span of free radicals. Mostly indirect methods focus on studying tissues affected by free radicals instead of direct observation and trapping of the latter. Here we present a method for the synthesis of biocompatible polymers based on polycyclic esters, in which fullerene C 60 , is chemically linked to the polymeric matrix. Fullerene is biocompatible and also known as a good radical scavenger capable to trap multiple small radicals from solutions. In such a way, radical trapping from living tissues and blood can happen on the surface of a biocompatible polymer which is in contact with physiological sample (for example as a filter built‐in into infusion device) allowing in‐vivo studying of free radical effects. In this project we first use the Diels‐Alder reaction to connect C 60 to amino‐anthracene. Next we use the Schiff‐base or reductive amination reactions of carbonyl groups of poly‐cyclic esters with primary amines in order to link the fullerene radical trap to the polymeric matrix. Like pure fullerene C 60 this biocompatible polymeric hook for free radicals may be used in tissues and physiological liquids in in‐vivo experiments. The synthesis, product isolation and characterization and preliminary trapping experiments will be presented. Grant Funding Source : Supported by Grant T34GM008411, from the National Institute of General Medical Sciences