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Acute aspiration of graphene sheets evokes transient airway hyperreactivity to methacholine in mice (660.4)
Author(s) -
Fedan Jeffrey,
Thompson Janet,
Sager Tina,
Roberts Jenny,
Fedan Jeffrey
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.660.4
Subject(s) - methacholine , bronchoconstriction , inhalation , basal (medicine) , medicine , chemistry , lung , endocrinology , in vitro , toxicity , anatomy , biochemistry , respiratory disease , asthma , insulin
Concern exists that the use of graphene sheets (GS) in composite materials might expose manufacturing workers to an inhalation hazard. Studies have shown that GS are cytotoxic in vitro (PC12 cells, fibroblasts) and in vivo in mice (lung granuloma), and Roberts et al. (2013) found non‐oxidized GS with larger lateral dimensions (蠅 5 μm) and a greater number of layers (~20) produced more lung inflammation up to 7 d after aspiration in mice when compared to smaller GS (<1 μm laterally, ~ 4 layers). The lung toxicity of various forms of GS has not been characterized completely. Here, we investigated the effects of GS on basal lung resistance (R L ), basal dynamic compliance (C Dyn ), and reactivity to inhaled methacholine (MCh) aerosol. Mice were given a non‐oxidized GS (5 μm x 5 μm laterally, 7 nm thick equal to ~ 20 layers; 40 µg) suspended in dispersion medium (DM; Porter et al., 2008) or DM (control) via aspiration. R L and C Dyn and reactivity to increasing concentrations of MCh aerosol were measured 4 h ‐ 2 mo after GS exposure. Basal R L was increased 4 h post‐exposure but at no other time; basal C Dyn was unaffected at any time. Airway reactivity to MCh (as ΔR L ) was increased at 4 h post‐exposure, and ΔC Dyn responses were decreased. GS was essentially without effect on R L or C Dyn at 1 d, 1 wk, 1 mo and 2 mo after administration. The results indicate that a single exposure to GS increases transiently lung resistance and reactivity to MCh. Grant Funding Source : Supported by NIOSH