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The role of GABA in hyperbaric oxygen‐induced acute antinociception in mice (659.16)
Author(s) -
Liu Shulin,
Shirachi Donald,
Quock Raymond
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.659.16
Subject(s) - nipecotic acid , nociception , pharmacology , antagonist , chemistry , gabaa receptor , bicuculline , anesthesia , medicine , neurotransmitter , receptor , biochemistry
Earlier we reported that mice responded with an acute antinociceptive effect during an 11‐min treatment with HBO 2 at 3.5 atmospheres absolute (ATA) (Ohgami et al ., NeuroReport 20:1325, 2009). We endeavored to explore the possible role of GABA in this HBO 2 ‐induced effect by pretreating different groups of male NIH Swiss mice, 20‐30 g, intracerebroventricularly (i.c.v.) or intrathecally (i.t.) with various drugs that influenced GABA neurotransmission. The pretreatment drugs were the GABA A antagonist SR 95531, the GABA B antagonist CGP 35348 and the GABA neuronal uptake‐inhibitor (±)‐nipecotic acid. Antinociceptive responsiveness to HBO 2 was assessed using the acetic acid‐induced abdominal constriction test conducted in a small animal hyperbaric chamber. HBO 2 at 3.5 ATA evoked a strong antinociceptive effect that was antagonized in dose‐related manner by i.c.v.‐ or i.t.‐administered SR 95531. I.c.v. and i.t. pretreatment with CGP 35348 or nipecotic acid potentiated HBO 2 ‐induced antinociception at higher doses. These data strongly suggest that GABA is involved in the acute antinociceptive response of mice to HBO 2 . Grant Funding Source : Supported by NIH Grant AT‐007222 and the Allen I. White Distinguished Professorship.