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Naringenin, a major bioactive lead of Thymus vulgaris , inhibits growth, induces apoptosis and modulates cell cycle and apoptosis‐regulatory gene expression in human cancer cells (657.18)
Author(s) -
Abaza Mohamed Salah,
Orabi Khaled,
AlAttiyah Raja'a,
AlQattan Ebtehal
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.657.18
Subject(s) - naringenin , apoptosis , cyclin dependent kinase 6 , cell cycle , cell growth , cancer research , chemistry , cell culture , biology , microbiology and biotechnology , cyclin , biochemistry , genetics , flavonoid , antioxidant
In the present study Thymus vulgaris was extracted in 96% ethanol then subjected to solvent/solvent partitioning processes and purification techniques. The identities of the isolated compounds, naringenin (Nar) and aromadendrin (Aro) were identified using different spectral techniques. Nar inhibited the growth of human colorectal, breast, and melanoma cancer cell lines in a dose‐ and time‐dependent manner, arrested their growth in S‐phase and G 2 /M‐phase or S‐phase and potently induced their apoptosis. Nar differentially down‐regulated the expression of Cdk4, Cdk6 , Cdk7, Bcl2, x‐IAP and c‐IAP‐2 , differentially up‐regulated the expression of p18 , p19 , p21, caspases 3 , 7 , 8 and 9 , Bak , AIF and Bax in both human colorectal and breast cancer cell lines. Nar could be a potential agent for prevention and/or treatment of human cancers. Grant Funding Source : this work was supported by Kuwait University, Research Grant No.[YS06/10]