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Phytochemical study and antinociceptive effect of the hexanic extract of leaves from Combretum duarteanum and friedelin in orofacial nociceptive models (657.14)
Author(s) -
Brito Renan,
Quintans Jullyana,
Costa Emmanoel,
Tavares Josean,
Estevam Charles,
Barison Andersson,
Silva Marcelo,
QuintansJúnior Lucindo
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.657.14
Subject(s) - nociception , friedelin , orofacial pain , capsaicin , chemistry , pharmacology , anesthesia , medicine , stereochemistry , biochemistry , surgery , receptor , triterpenoid
We investigated the antinociceptive effects of the hexanic extract (HE) of leaves from C. duarteanum and friedelin, its mainly compound, in formalin‐, glutamate‐ and capsaicin‐ induced orofacial nociception models. In order to isolate of friedelin from HE, flash chromatography technique was used. Male Swiss mice (n=8/group) were pretreated with HE (100, 200 or 400 mg/kg; p.o.), friedelin (50 mg/kg, p.o.), vehicle (Tween 80 0.2%; p.o.) or morphine (5 mg/kg; i.p.) before the injection of algogen agents into the right upper lip (perinasal area). The test of formalin‐induced orofacial nociception showed that HE and friedelin significantly reduced nociception (p<0.001), in both phases of testing, when compared with the vehicle. In the glutamate and capsaicin‐induced orofacial nociception tests, pre‐treatment with HE produced a significant orofacial anti‐nociception (p<0.001), at all doses tested, when compared with the control group. The results suggest the HE and friedelin possess antinociceptive effects in models of orofacial nociception in rodents. Grant Funding Source : Suported by CAPES/Brazil, CNPq/Brazil and FAPITEC/Brazil