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Electrically monitoring effects of cisplatin on transendothelial invasion of human ovarian cancer cells (655.11)
Author(s) -
Lo JunChih,
Lo ChunMin
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.655.11
Subject(s) - cisplatin , ovarian cancer , umbilical vein , metastasis , cancer cell , cancer research , medicine , cancer , chemotherapy , cell , cell culture , chemistry , biology , in vitro , biochemistry , genetics
Cisplatin is a platinum based chemotherapy drug used to treat various types of cancers, including sarcomas, some carcinomas, lymphomas and germ cell tumors. In this study human ovarian cancer cell lines, OVCA429 and ALST, were challenged with different concentrations of cisplatin (1E‐6M, 1E‐5M, 5E‐5M and 1E‐4M) and the effects on cancer cell morphology and micromotion were monitored and analyzed by electric cell‐substrate impedance sensing (ECIS) and microscopy. Moreover, the transendothelial invasion of the ovarian cancer cells to human umbilical vein endothelial cells (HUVECs) was examined. Our measurements showed that 1E‐4M of cisplatin caused a decrease of junctional resistance between cells and the reduction in micromotion. In addition, 1E‐6M and 1E‐5M of cisplatin were not toxic to the integrity of HUVEC layers but effectively inhibited transendothelial invasion of ovarian cancer cells. Thus, we report a strategic approach for using ECIS to analyze potential drugs for their abilities to suppress metastasis, particularly at the level of transendothelial invasion. Grant Funding Source : Supported by the National Science Council in Taiwan