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Orpin and its isoform are novel putative calcium binding proteins associated to intestinal and nerve regeneration in the sea cucumber Holothuria glaberrima (651.22)
Author(s) -
SotoAcaba Arisnel,
OrtizPineda Pablo,
HernandezPasos Josue,
GarciaArraras Jose
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.651.22
Subject(s) - regeneration (biology) , gene isoform , biology , microbiology and biotechnology , signal peptide , calcium binding protein , extracellular matrix , calcium , gene , biochemistry , chemistry , peptide sequence , organic chemistry
Holothuria glaberrima exhibits the capacity to regenerate body parts after injury or loss. This ability is mediated by an initial blastemal formation which requires proliferation and reorganization of precursor cells. Accordingly, various molecules play key roles in different aspects of the regeneration of a body part. The use of microarrays and high‐throughput sequencing lead our laboratory to identify a series of new molecules associated to different processes of regeneration. Such is the case for the protein Orpin. Based on an EST database, we found that Orpin gene is over expressed at early stages of intestinal regeneration. Its expression occurs concomitantly with remodeling of the extracellular matrix. Also, another isoform was identified and both sequences were present in libraries from regenerating nerve and intestinal tissues. The characterization between those sequences indicates that both encode two EF‐hand motifs. Unlike other known calcium binding proteins, they exhibit a signal peptide. The use of Orpin antibodies showed the labeling of the luminal epithelium as well as the radial nerve. Moreover, these antibodies are being used to determine the tissue and temporal specificity of Orpin and its isoform. In order to evaluate the effect of Orpin during regeneration, two His‐tagged fusion proteins are expressed to be injected into regenerating animals. Thus, determining the role of Orpin and its isoform during the regeneration process would help to elucidate the molecular mechanisms involved in different aspects of nerve and intestinal regeneration. Grant Funding Source : NIH (1SC1GM084770)