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Electrospun fiber mat design based on brain development: a new model for neural progenitor migration (651.17)
Author(s) -
Czeisler Catherine,
Short Aaron,
Gygli Patrick,
Ortiz Cristina,
Catacutan Fay,
Winter Jessica,
Otero José
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.651.17
Subject(s) - extracellular matrix , progenitor cell , scaffold , neural stem cell , progenitor , chemistry , microbiology and biotechnology , biomedical engineering , anatomy , biophysics , biology , stem cell , engineering
The brain is a complex organ made up by cells that are born far away from their adult positions and thus migrate large distances to form connections during development. This migration has been mostly studied on the scaffold formed by the Radial Glia at the germinal zones. However, we discovered that during normal embryonic development, migratory neural progenitors use other scaffolds such as blood vessels and the pial surface in order to migrate these large distances. Further, these different scaffolds contain different Extracellular Matrix (ECM) molecules, and the ECM composition atop the scaffolds changes dynamically during development. We hypothesize that the migration of neural progenitors is influenced by the topology (shape) as well as molecular composition (ECM) of these different scaffolds. In order to illustrate this phenomenon, we have modeled the three principal scaffolds in the brain (radial glia, pial surface and blood vessels) out of electrospun fibers. These fibers can be formed at different diameters to mimic a blood vessel (10‐15um) or radial glial process (3‐5um) topology or a flat tortuous surface like the pial surface, and they can be coated with different ECM proteins corresponding to the in vivo data. We cultured Neural Stem Cells (NSC) on top of these fibers and observed their migratory behavior. Grant Funding Source : NCATS Grant 8UL1TR000090‐05