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Antimicrobial peptide and mucin expression differ between the diabetic NOD mouse and non‐diabetic NOR mouse (650.16)
Author(s) -
Daft Joseph,
Lorenz Robin
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.650.16
Subject(s) - nod mice , nod , mucin , ileum , biology , immune system , endocrinology , firmicutes , gut flora , mucin 2 , islet , antimicrobial peptides , medicine , immunology , diabetes mellitus , gene expression , microbiology and biotechnology , antimicrobial , gene , biochemistry , 16s ribosomal rna
Type 1 Diabetes (T1D) is defined as the selective immune destruction of insulin producing β‐cells within the islet. Alterations in the intestinal microbiota, increased intestinal permeability, and an aberrant immune system are thought to play key roles in the development of T1D. Recent studies have shown that mice that develop T1D have a different microbiota compared to mice that do not develop T1D. However why mice that are on the same diet in the same facilities have different microbiota is unknown. We hypothesize that altered antimicrobial peptide (AMP) and mucin production early in life determines ones microbiota, protecting some, but not others from T1D. Non‐obese diabetic (NOD) female mice were compared to age and sex matched Non‐obese diabetic resistant (NOR) mice. AMP and mucin gene expression was measured in the ileum and colon of the two strains and protein expression was examined by immunohistochemistry. At 2 weeks of age there is a significant increase in the AMP, defcr‐4, in the ileum of NOD mice compared to NOR mice. In addition a decrease in the mucins, Muc 1, 2, and 3 was measured in the colons of NOD mice compared to NOR mice. This correlates with alterations that we have measured in Bacteroidetes and Firmicutes between the two strains of mice. We postulate that these differences in AMP and mucin expression between the two strains leads to alterations in microbiota, which leads to differences in disease outcome.