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Influences of fermentation period on anticancer effects of Shuidouchi in MCF‐7 human breast adenocarcinoma cells (647.3)
Author(s) -
Zhao Xin,
Qian Yu,
Li Guijie,
Song JiaLe,
Wang Qiang,
Sun Peng
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.647.3
Subject(s) - fermentation , chemistry , food science , daidzein , isoflavones , mcf 7 , gtp' , biochemistry , genistein , human breast , biology , breast cancer , cancer , medicine , enzyme , endocrinology
Shuidouchi (SDC) is a kind of short time‐fermented soybean food. Based on the SDC made through different fermenting time, the relationship between physicochemical properties and in vitro anticancer effects were evaluated by pH, acidity, total viable count, amino and ammonia type nitrogens, activity of γ‐GTP, sensory evaluation, MTT and RT‐PCR analysis. As the fermentation time increased, the pH and the total viable count increased in SDC. SDC fermented for 48 hour demonstrated the highest content of acidity and γ‐GTP activity. The ammonium nitrogen and amino acid nitrogen contents of 48 h fermented SDC were a little lower than 60 h fermented SDC, but much higher than 24 h fermented SDC. The 48 h fermented SDC also appeared the highest content of daidzein and genistein. At 2 mg/mL concentration, the growth inhibitory rate of 48 h fermented SDC was 78.8% higher than that of sea 24 h and 60 h fermented SDC in MCF‐7 human breast adenocarcinoma cells. The low concentration (1 mg/mL) of 24 h, 48 h and 60 h fermented SDC showed the similar growth inhibitory effect to 2 mg/mL SDC treatment at 22.5%, 43.5% and 35.2%, respectively. 2 mg/mL of 48 h fermented SDC treated MCF‐7 cells increased the Bax, caspase3,9 genes, decreased the Bcl‐2 gene compared with the other periods fermented SDC. From the results, we concluded that the 48 h fermented SDCZ showed the best physicochemical quality and in vitro anticancer effects.