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Flavonoids from rooibos tea promote cell death in an osteosarcoma cell line (647.10)
Author(s) -
Nash Leslie,
Ward Wendy
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.647.10
Subject(s) - chemistry , vitexin , formazan , luteolin , viability assay , cell culture , cell growth , pharmacology , biochemistry , antioxidant , cell , flavonoid , biology , genetics
The Saos2 cell culture system is a well characterized osteoblast‐like cell line used to assess the effects of dietary components such as flavonoids on the production of bone mineral. Saos2 cells are derived from human osteosarcoma and possess desirable characteristics for assessing molecular interactions and mechanistic routes in which nutrients may influence mineral production. While screening rooibos tea flavonoids for potential positive effects on bone mineralization, several flavonoids reduced mineralization, while exhibiting cellular death. Mineral, measured using an Alizarin red assay, was reduced in cells treated with isoquercetin (蠅10uM, p<0.001) vitexin (蠅5uM, p<0.05) and luteolin (蠅15uM, p<0.001) in comparison to controls. Metabolic activity of the mitochondrial reductase was analyzed over 72 hours as a marker for cell viability by measuring the reduction of a tetrazolium dye to a formazan salt. The addition of isoquercetin (p<0.001), vitexin (p<0.001) or luteolin (p<0.001) was shown to attenuate mitochondrial activity in a concentration dependent manner, and was in agreement with the mineralization data. Human osteosarcoma is known for its ability to resist chemotherapy. Thus, further investigation of these compounds may help develop natural strategies that enhance chemotherapeutic agents in addition to reducing toxic side effects.Grant Funding Source : Supported by a NSERC Discovery Grant to W. Ward