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Astaxanthin ameliorates UVB‐induced inflammation in keratinocytes and keratinocyte‐dependent MMP‐1 production in fibroblasts in vitro (645.2)
Author(s) -
Yamashita Eiji,
Hongo Nobuko,
Tominaga Kumi
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.645.2
Subject(s) - keratinocyte , astaxanthin , cosmeceutical , matrix metalloproteinase , hacat , chemistry , human skin , in vitro , inflammation , melanin , photoaging , cell culture , microbiology and biotechnology , immunology , biology , medicine , dermatology , biochemistry , pathology , genetics , carotenoid
Recently, astaxanthin has been reported to have the cosmeceutical benefits for human skin. To investigate the mechanism of action we examined the effects of astaxanthin on the UVB‐induced production of inflammatory cytokines and mediators in human epidermal keratinocytes and on the keratinocyte‐dependent induction of matrix‐metalloproteinase (MMP)‐1 in human dermal fibroblasts in vitro . As the results, astaxanthin treatment significantly inhibited the production of IL‐1α, IL‐6, IL‐8, TNF‐α, PGE 2 and POMC induced by UVB irradiation in the keratinocytes. The conditioned media from UVB irradiated keratinocyte culture significantly increased the production compared to the media from non‐UVB culture. The increased production of MMP‐1 treated by the conditioned media from UVB irradiated keratinocyte culture was significantly inhibited by the supplementation of conditioned media from UVB irradiated keratinocyte culture with astaxanthnin treatment. The inhibition was in a dose dependent manner. It suggests that astaxanthin ameliorates UVB‐induced inflammation in keratinocytes resulting in the inhibition of UVB‐induced damage such as melanin production and wrinkle formation in not only epidermal keratinocytes but dermal fibroblasts of the skin.