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Extraction and normal‐phase separation of brain carotenoids and tocols (645.10)
Author(s) -
Craft Neal
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.645.10
Subject(s) - saponification , xanthophyll , lutein , chemistry , zeaxanthin , chromatography , carotenoid , extraction (chemistry) , hexane , beta carotene , solvent , biochemistry
Tocopherols and carotenoids have been implicated in reduced risk of Alzheimer’s Disease and improved cognition. Aim: To develop an improved extraction of brain and normal‐phase separation to measure 4 tocopherols, 4 tocotrienols (T3) and xanthophylls and to avoid saponification. Methods: Brain tissue was extracted with several solvent combinations including SDS, MTBE, hexane, and THF. The extracts were separated using a Diol column with a gradient of hexane and dioxane and included two internal standards (Tocol and B‐apo8’ carotenoate). Carotenoids were measured at 450nm and tocols by fluorescence (296nm ex/340nm em). Results: No single solvent provided the best extraction of all the antioxidants. An initial MTBE extraction was followed by hexane/THF. Tocopherol recoveries ranged from 96‐103% with CVs of 6‐25% at sub‐ppm and xanthophylls at ppb levels. Four tocopherols, 4 T3s, a‐ and B‐cryptoxanthin, lutein and zeaxanthin were measured in 0.1g of human brain. Conclusions: A selective and sensitive method was developed to measure 8 tocols and several xanthophylls in 0.1g of brain without saponification.