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Stichopus japonicus acid mucopolysaccharide inhibits growth of human liver cancer cells by modulating mitochondrial apoptosis pathway (644.15)
Author(s) -
Song Yang,
Chen Chen,
Jin Yang
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.644.15
Subject(s) - survivin , apoptosis , mitochondrion , flow cytometry , microbiology and biotechnology , cell , biology , inner mitochondrial membrane , chemistry , biochemistry
Sea cucumber has traditionally been used as medicinal food for promoting health and warding off disease. Stichopus Japonicus Acid mucopolysaccharide (SJAMP) isolated from sea cucumbers have been shown to have anti‐tumor property. Several working mechanisms are proposed to explain SJAMP’s anti‐tumor effect. In this study we investigated whether SJAMP exerts its anti‐tumor effect via the mitochondrial apoptosis pathway in a cell‐based model. After incubating human liver cancer cell line Hep G2 cells with SJAMP at 0.25, 1, and 4 μg/mL for 24 h, we examined, using the indicated methods, cell morphology (transmission electron microscopy), mitochondrial membrane potential (flow cytometry), and expression of gene (real time RT‐PCR) and protein (immunohistochemistry) of apoptosis‐related molecules. Results showed that SJAMP treatment significantly reduced mitochondrial membrane potential, down‐regulated gene and protein expression of anti‐apoptotic Bcl‐2 and Survivin ( P <0.05), and up‐regulated gene expression of pro‐apoptotic Bax, CytC, Caspase‐3, and Smac ( P <0.05). These findings indicate that SJAMP may inhibit growth of Hep G2 cells by promoting mitochondria‐mediated apoptosis pathway. Grant Funding Source : Supported by National Natural Science Foundation