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Nutrient excess promotes accumulation of bone marrow‐derived progenitor cells in adipose tissue (641.12)
Author(s) -
Holden Candice,
Wysoczynski Marcin,
Sansbury Brian,
Hellmann Jason,
Zafar Nagma,
Gibb Andrew,
Bhatnagar Aruni,
Hill Bradford
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.641.12
Subject(s) - adipose tissue , stem cell , bone marrow , progenitor cell , adipocyte , endothelial stem cell , endocrinology , adipose tissue macrophages , biology , medicine , bone marrow stem cell , microbiology and biotechnology , white adipose tissue , immunology , in vitro , biochemistry
Obesity has been implicated as a major risk factor for the development of several chronic diseases, including Type 2 diabetes. Because adipose tissue (AT) must remodel remarkably to accommodate lipid storage under conditions of nutrient excess, we hypothesized that AT stem cells are essential for adipose organ expansion. Therefore, we first sought to determine the source of AT stem cells and to measure changes in AT stem cell populations that occur with obesity. To delineate the contribution of bone marrow (BM)‐derived cells, mice were irradiated and the BM was repopulated with GFP+ donor cells. The mice were then placed on low‐fat (LF) or high‐fat (HF) diets for 12 weeks and changes in inflammatory cell populations and AT stem cells were measured. F4/80+ macrophages increased 2.5‐fold in the visceral AT with 12 weeks of HF diet. Similarly, BM‐derived Sca‐1+/CD29+/CD24+ cells—putative adipocyte stem cells shown to have the capacity to form adipocytes in vivo— increased 2.8‐fold in the AT of mice fed a HF diet. Sca‐1+/Flk+ endothelial progenitor cells also increased 2.6‐fold in the visceral AT. These data suggest that adipocyte and endothelial stem cell populations derive from bone marrow. Their recruitment to adipose tissue could be important for AT remodeling under conditions of caloric excess.