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Characterization of the Loucy cell line as a model for human peripheral γδ T cells (638.5)
Author(s) -
Dai Xiaoshuang,
Stanilka Joy,
Percival Susan
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.638.5
Subject(s) - interleukin 21 , il 2 receptor , peripheral blood mononuclear cell , cytotoxic t cell , natural killer t cell , antigen presenting cell , t cell , cd40 , cd8 , microbiology and biotechnology , zap70 , biology , immunology , immune system , in vitro , biochemistry
As unique innate‐like lymphocytes, γδ T cells make up a small proportion of circulating T cells. Up to now, there is no widely accepted cell line model for human γδ T cell. Our aim was to investigate whether Loucy cell line shares similarities with γδ T cells derived from human peripheral blood mononuclear cells (PBMC). First, 95% of Loucy cells express the unique γδ T cell receptor (TCR). Second, most Loucy cells were double negative for CD4 and CD8, like most peripheral γδ T cells. Third, Loucy cells express TLR2, like peripheral γδ T cells. At the functional level, Loucy cells proliferated in response to isopentenyl pyrophosphate, a known phosphoantigen for γδ T cell proliferation. Activated Loucy cells express CD69 on cell surface, similar to γδ T cells in activated mixed PBMC cultures. Unlike peripheral γδ T cells, Loucy cell did not express NKG2D. TCR Vδ gene usage in Loucy cells is mainly Vδ3 and to a lesser extent Vδ1, while Vδ2Vγ9 T cells dominate in blood. In addition, 95% of Loucy cells were CD45RO+ (memory), whereas γδ T cells in PBMC were 45% CD45RO+ and 52% CD45RA+ (naive). The only cytokine detected from PHA‐L‐stimulated Loucy cells was TGF‐β, whereas peripheral γδ T cells produce many more cytokines. These results suggest that Loucy cells have many characteristics similar to γδ T cells in PBMC, and this cell line, for some aspects of γδ T cell function, may be useful as an in vitro model for human γδ T cells. Grant Funding Source : Funded in part by UF AES