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Insights into infant nutrition through cross‐species proteomic comparison of primate milks (623.9)
Author(s) -
Beck Kristen,
Weber Darren,
Phinney Brett,
Smilowitz Jennifer,
Korf Ian,
Lemay Danielle
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.623.9
Subject(s) - rhesus macaque , macaque , proteome , biology , proteomics , primate , immune system , computational biology , immunology , physiology , bioinformatics , biochemistry , neuroscience , gene
Milk has served as the optimal neonatal nutrition source directing infant growth, neurological development, and immunity for millions of years. Its production is an energetic balance between mother and infant needs. Therefore, molecular components providing a competitive advantage against genetically similar peers will be retained. Milk proteins have alternative end points aside from being an amino acid source. Bioactive peptides have antimicrobial and immunmodulatory functions. Recent studies show milk proteins to vary in abundance with disease status. Further biomedical research relies on non‐human models, specifically the rhesus macaque. Currently, little is known about the rhesus macaque milk proteome due to prohibitive small volume of samples collected. Our group developed a small sample method for quantitative milk proteomics using LC‐MS/MS. We identified over 800 proteins in macaque milk (protein and peptide FDR = 4.4%, 0.9%, respectively). In our initial analysis, proteins associated with assisting the innate immune response (complement proteins C3, C4, factors B, H, and I) are more abundant but lysozyme C is less abundant in macaque compared to human milk. By understanding the rhesus macaque milk proteome, we can better utilize this model organism for investigation of health related diagnostic tools. Cross‐species comparisons will also highlight which milk proteins are unique to human nutrition. Grant Funding Source : K.B. supported by T32‐GM008799 from NIGMS‐NIH and CNPRC Post‐Doc Pilot to DL NIH/NCRR P51 RR00169