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Proteomic fingerprinting of intestinal epithelial cell Caco‐2: insights into molecular characteristics during proliferation and differentiation (623.20)
Author(s) -
Cundiff Judy,
Maria Sarah,
Lohe Kimberly,
McMahon Robert,
Zhang Qiang
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.623.20
Subject(s) - enterocyte , adherens junction , microbiology and biotechnology , biology , caco 2 , proteome , stable isotope labeling by amino acids in cell culture , intestinal epithelium , intracellular , extracellular , endocytosis , brush border , cell , proteomics , epithelium , small intestine , biochemistry , cadherin , genetics , gene , vesicle , membrane
Caco‐2 cells are widely used as an in vitro model to study mechanisms in enterocyte development and intestinal epithelial transport. The cells spontaneously differentiate into enterocyte phenotypes after confluency. Nevertheless, a systemic description of the molecular signatures and underlying mechanisms during Caco‐2 development remain largely undefined. We applied quantitative proteomic data at various stages of Caco‐2 proliferation and differentiation (days post‐seeding: 3, 7, 12, 16, 22 and 25) as probes for enterocyte development. 3781 proteins were identified where 2310 are with quantitative value(s). Levels of adherens and tight junction proteins increase up to day 12 and become steady afterwards whereas the levels of cell cycle proteins decrease after day 12, suggesting additional factors to account for transepithelial electrical resistance which continues to increase from day 7 to 22. Expression of intestinal sucrase isomaltase increases from day 7 to 16 and remains stable afterwards, indicating the maturation of intestinal brush border. The abundance changes of marker proteins in extracellular matrix formation, endocytosis, leukocyte transepithelial transport and various intracellular processes were also determined. Insights from the developing Caco‐2 proteome may be used to assess various nutritional treatments during early intestine development.