Premium
Comparison of commercially‐available methods to preserve DNA in human milk (623.10)
Author(s) -
Lackey Kimberly,
Williams Janet,
Mosely Janae,
Brooker Sarah,
McGuire Mark,
McGuire Michelle
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.623.10
Subject(s) - preservative , food science , dna , chemistry , saliva , biochemistry
Sequencing methods used to characterize the milk microbiome require that milk DNA content not change between time of collection and analysis. Although cold storage is optimal, chemical preservatives are needed when refrigeration is not available. The objective of this study was to test the efficacy of 4 commercially‐available preservatives ‐ RNAlater (RNAL, Ambion), Milk Preservation Solution (MPS, Norgen Biotek), DNAgard Saliva (SAL, Biomatrica), and Broad Spectrum Microtabs II (TAB, D&F Control Systems) ‐ in milk held at 37 °C for 6 wk. Aliquots of 8 pools of fresh human milk were treated with each preservative; DNA was extracted immediately and 1, 2, 4, and 6 wk later. DNA was quantified using the Qubit dsDNA HS Assay (Life Technologies). There was a treatment by week interaction (P<0.05) on milk DNA content. After an initial decrease, DNA concentration of MPS‐treated milk remained constant, whereas that of milk treated with SAL, RNAL, or TAB did not change. However, more DNA was consistently extracted using MPS or SAL (P<0.05) than with RNAL or TAB. In summary, preliminary evidence suggests MPS and SAL may be suitable to preserve DNA in milk for up to 6 wk at high ambient temperatures.