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Evaluating sodium and potassium measuring technologies for human milk (622.1)
Author(s) -
Hartmann Peter,
Geddes Donna,
Lai Ching Tat
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.622.1
Subject(s) - chemistry , potassium , hydrogen peroxide , sodium , nitric acid , inductively coupled plasma atomic emission spectroscopy , analytical chemistry (journal) , ion , inductively coupled plasma , chromatography , inorganic chemistry , plasma , biochemistry , physics , organic chemistry , quantum mechanics
Sodium (Na) and potassium (K) in human milk are associated with the permeability of the lactating breast. We hypothesized that the measurement using an ion selective electrode is similar to inductively coupled plasma atomic emission spectroscopy (ICP‐AES) that requires very low sample volumes. Four groups (low to high fat) of milk samples (n= 30 per group, analyzed in duplicate) were warmed and homogenized. An aliquot (0.3 mL) was used to determine [Na] and [K] by the ion selective electrodes (HORIBA, Japan). Another aliquot (0.01 mL) was digested with nitric acid/hydrogen peroxide and used to determine [Na] and [K] by ICP‐AES (Thermo Scientific, USA). Linear mixed models were used to test whether differences between the two techniques were significantly different from zero for the full data set. Although the measurements using both technologies were within published values, measurement by ICP‐AES for Na and K were (mean mM±SD) 9.8±5.0 and 11.0±4.8,, respectively, which were higher (p<0.05) than by ion selective electrode (5.8±1.5 and 9.1±3.1, respectively). This might due to the complete release of Na and K ions of human milk by the nitric acid/hydrogen peroxide digestion. Therefore, a conversion factor can be applied to the data to allow comparison of values obtained using these two techniques. Grant Funding Source : Medela, AG