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Impacts of erythropoietin combined with granulocyte‐colony stimulating factor on neural function after traumatic brain injury in rats (618.6)
Author(s) -
Duan Hubin,
Hao Chunyan,
Li Shuzhen,
Lu Gui,
Fan Yimin
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.618.6
Subject(s) - erythropoietin , neun , traumatic brain injury , medicine , granulocyte colony stimulating factor , group a , group b , endocrinology , immunohistochemistry , chemotherapy , psychiatry
Objective: To investigate the effect of erythropoietin (EPO) and granulocyte‐colony stimulating factor (G‐CSF) combination therapy on functional recovery and nerve cell regeneration after TBI. Methods: The rats model with TBI was prepare by Feeney’s methods. 100 male Wistar rats were randomly divided into Sham operation group(Sham group), traumatic brain injury group (TBI group), EPO treatment group(T+E group ), G‐CSF treatment group(T+G group), and EPO combined G‐CSF group(T+E+G group), and then each group was sub‐divided into 5 subgroups according to the time 1,3 ,5,7 and 14 days after TBI.(Each subgroups n=4). Each treatment group rats were immediately given corresponding intervention measures after TBI for 3 days. Neurological function was assessed using a modified neurological severity score(mNSS) before rats were sacrificed at 1,3,5,7 and 14 days post TBI . Immunofluorescence measurement was used to detect newly proliferation cells number and its direction of differentiation by BrdU single positive cells and BrdU/NeuN or BrdU/GFAP double positive cells. Results: The mNSS scores of each treatment group was significantly lower than TBI group, and T+E+G group was also significantly lower than T+E group or T+G group at 5 ,7 and 14 days of surgery (P < 0.05); The BrdU positive cells number in T+E+G group was significantly higher than other groups starting from 3 days after TBI, and the peak at 7 days (P < 0.05). Compare the groups of brain tissue BrdU/NeuN and BrdU/GFAP double positive cells number, T+E+G group were significantly higher than other groups, T+E group and T+G group were significantly higher than Sham group and TBI group (P < 0.05). Conclusions: Combined use of EPO and G‐CSF can obviously improve functional recovery and promote the brain nerve cell regeneration differentiating to neurons and astrocytes in the injury boundary zone. Grant Funding Source : This study was supported by the National Natural Science Foundation Youth (Fund No.30600637)