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Metabolic profile of fragrant and non‐fragrant ornamental potato flowers (617.5)
Author(s) -
Lawrence Jim,
Sauer Jon
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.617.5
Subject(s) - phenylacetaldehyde , population , ornamental plant , odor , horticulture , food science , biology , chemistry , botany , biochemistry , medicine , environmental health , neuroscience
Odoriferous floral organic compounds are important biological signaling agents. These compounds are often sought for their pleasant characteristics in perfumery and gardening. Wild samples of ornamental potato flower possessing an unexpected rose‐resembling odor (P population) were compared to unscented potato flowers of close relation (VER Population). Volatile small‐molecule analysis by GC/MS, coupled with spectral identification software, identified three volatiles connected to fragrance: β‐farnesol, phenethyl alcohol (PEA), and phenylacetaldehyde (PA). Standards of each compound were run to ensure proper identification by comparing retention time as well as fragmentation patterns. Additionally, concentrations of PA and PEA were obtained. Elevated PEA concentrations in the P population were found to accompany depressed PA. Conversely, decreased PEA was found in the VER population with increased PA. Phenacetaldehyde reductase (PAR), responsible for the conversion of phenylacetaldehyde reductase to phenethyl alcohol, is now being investigated using SDS‐PAGE with LC/MS. Preliminary results in SDS‐PAGE show that differential protein expression exists between the P and VER populations. Determining whether PAR is the culprit opens up characterization of the genotype of the P population. These findings can be important for designer plant breeding seeking to incorporate fragrant phenotypes. Grant Funding Source : Supported by UWSP College of Letters and Science