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Evaluating multiple signaling activities in LPS‐induced human peripheral blood mononuclear cells (613.6)
Author(s) -
Martin Ashley,
Kohlmeier Kayla,
Maas Lindsay,
Gautam Aarti,
Jett Marti,
Mendis Chanaka
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.613.6
Subject(s) - signal transduction , peripheral blood mononuclear cell , lipopolysaccharide , gene expression , microbiology and biotechnology , biology , microarray analysis techniques , gene , septic shock , microarray , immunology , sepsis , in vitro , biochemistry
Lipopolysaccharide (LPS) is an endotoxin that induces the secretion of pro‐inflammatory cytokines in many cells; and LPS has been well characterized to induce septic shock in humans. This project is aimed at characterizing the effects of LPS by utilizing signal transduction pathways induced by LPS in Peripheral Blood Mononuclear Cells (PBMC). A set of LPS induced genes were identified through microarray analysis and common signaling pathways were identified. Key pathway inter‐connectors such as JNK and p38 were blocked and were evaluated to the previously identified gene expression patterns. The main objective was to investigate the effect of two inhibitors on the time dependent gene expression pattern and then, the impact on the components that are indirectly associated with the inhibited signaling pathways. In addition, the specific inhibitors were evaluated by correlating the observed gene expression pattern to the protein expression pattern. We anticipate that the study will allow us to better understand the complex interactions of multiple signal transduction pathways induced by LPS as well as provide information about mitigating the deadly effects of LPS.