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Eps8 protein participates in migration in Toll‐like receptor‐engaged macrophages (609.7)
Author(s) -
Hsieh Ming Yu,
Chen YenJen,
Chang Miao Ying,
Lee Chung Ta,
Lee JenqChang,
Maa MingChei,
Leu TzengHorng
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.609.7
Subject(s) - motility , proto oncogene tyrosine protein kinase src , tlr4 , microbiology and biotechnology , phagocytosis , signal transduction , macrophage , receptor , biology , chemistry , biochemistry , in vitro
Previously, we demonstrated that iNOS/Src/FAK axis was a general mechanism of macrophage motility in response to various pathogen‐associated molecular patterns (PAMPs) and Eps8 participated in TLR4‐mediated signal transduction leading to enhanced phagocytosis and bacterial killing effect. However, the role of Eps8, an Src‐interacting protein, in PAMP‐mediated macrophage locomotion is still unclear. Here we observed that the expression of Eps8 was PAMP‐inducible, and the induction was iNOS/Src‐dependent. Attenuation of Eps8 simultaneously impaired Src activity and suppressed macrophage mobility. Remarkably, ectopic Eps8 could partly restore the reduced motility and Src activity in Src‐attenuated macrophages exposed to various PAMPs. Together, our findings indicated that Eps8 not only modulated TLR4‐mediated signal transduction but also participated in Src‐mediated cell migration in TLRs‐stimualted macrophages.