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O‐GlcNAc modulates β‐catenin localization during M‐phase in HeLa cells (607.18)
Author(s) -
Lucena Miguel,
Hu Ping,
Todeschini Adriane,
Han Guanghui Han,
Dias Wagner,
Hart Gerald
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.607.18
Subject(s) - midbody , mitosis , anaphase , cytokinesis , microbiology and biotechnology , chemistry , catenin , hela , cytoplasm , wnt signaling pathway , cell cycle , metaphase , cell , biochemistry , signal transduction , cell division , biology , chromosome , gene
β ‐catenin is already known to be O‐GlcNAcylated. However, the function of this PTM is not well established. Here, we show that increased O‐GlcNAc levels induced the interaction with OGT, providing the accumulation of glycosylated form of β ‐catenin in cytoplasm. In addition, the WNT treatment rapidly decreased the O‐GlcNAc levels indicating an interplay between this PTM and the canonical pathway. Further, we showed that β‐catenin co‐localized with OGT and OGA during the mitosis. However, a strong co‐localization with OGA was observed during the metaphase specially with centrossome. Surprisingly, the β‐catenin strongly co‐localized with OGT at anaphase within the central spindle. In addition, the co‐localization of β‐catenin with OGT seems to be stronger at the midbody during cytokinesis when compared with OGA. Taken together, our date indicates that O‐GlcNAcylation of β‐catenin modulates its sub‐cellular localization during cell cycle. Grant Funding Source : CNPq, CAPES, FAPERJ