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Phosphatidylethanolamine increases perilipin 2 binding to synthetic lipid droplets (606.5)
Author(s) -
Rickertsen Cassandra,
Townsend Elizabeth,
Listenberger Laura,
Kharbanda Kusum
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.606.5
Subject(s) - perilipin , phospholipid , lipid droplet , phosphatidylethanolamine , phosphatidylcholine , chemistry , biochemistry , lipolysis , adipose tissue , membrane
Excess lipid is stored in organelles known as lipid droplets. Each lipid droplet is composed of a neutral lipid core surrounded by a phospholipid monolayer. The surface of each lipid droplet is decorated with proteins that facilitate fat storage and utilization. Our lab studies perilipin 2, a protein that binds to the surface of lipid droplets and slows the degradation of stored fat. We aim to understand how the phospholipid composition of the lipid droplet influences perilipin 2 binding. Here, we show that perilipin 2 binding to synthetic lipid droplets is influenced by the phospholipid composition of the surface. Specifically, perilipin 2 binding decreases as phosphatidylcholine (PC) levels decrease relative to phosphatidylethanolamine (PE). This result may influence the progression of alcohol‐induced fatty liver disease. Our experiments show that alcohol consumption in rats not only increases hepatic triacylglyceride storage and lipid droplet accumulation but also alters the phospholipid composition of hepatic lipid droplets. We observe a decrease in the PC:PE ratio of hepatic lipid droplets following alcohol consumption. Future experiments will continue to explore the link between the lipid droplet phospholipid composition, perilipin 2 binding, and alcohol‐induced fatty liver disease. Grant Funding Source : Supported by a grant to St. Olaf College from the Howard Hughes Medical Institute through the Precol