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Lipid‐dependent proliferation of breast and pancreatic cancer cell lines (605.7)
Author(s) -
Pyrek Sebastian,
Fahmi Tariq,
Saeed Lamya,
Biris Alex,
RadominskaPandya Anna
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.605.7
Subject(s) - lipid metabolism , nile red , lipid droplet , pancreatic cancer , cancer cell , cell culture , oil red o , cell growth , apoptosis , chemistry , cell , biochemistry , biology , cancer , microbiology and biotechnology , in vitro , physics , adipogenesis , quantum mechanics , fluorescence , genetics
Cancer cells rely heavily on anaerobic glycolysis for energy production, and have the capacity to synthesize their own sup¬ply of biologically active compounds of predominantly lipid origin. We hypothesize that any perturbation of this lipid pool, caused by drug treatment or changes in lipid metabolism, will result in significant alterations in the biological processes of these cells and result in cell death. In these studies, the lipid content of breast and pancreatic cell culture models has been assessed. Staining with Nile Red and Oil Red O indicate that primary cancer cell lines contain significantly higher lipid levels than metastatic lines and that the subcellular localization of these lipids also varies. Separation, identification, and quantitation of total lipid extracts by TLC and LCMS indicates that the proportions of phospholipids also vary in relation to the level of tumor cell differentiation. Treatment with drugs and transfection of cells with plasmids for specific UGT isoforms, which are known to conjugate various bioactive lipids and to be down regulated in several cancers, affects the lipid content and results in decreased cell proliferation and death. Nanodelivery of these drugs and plasmids significantly enhances these effects. Examining the role of lipids in cancer cell homeostasis is a novel approach to cancer research. Grant Funding Source : Supported By DoD‐W81XWH1110795 and UAMS TRI ABCRP