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Isolation and genomic annotation of two novel bacteriophage known to infect Mycobacterium smegmatis (589.5)
Author(s) -
Paschalis Valerie,
Patel Shivani,
Aziz Froogh,
Chen Alston,
Monsen Kirsten,
Adams Sandra,
Vega Quinn
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.589.5
Subject(s) - mycobacterium smegmatis , biology , bacteriophage , genome , mycobacterium tuberculosis , microbiology and biotechnology , mycobacterium , phage therapy , genbank , gene , genetics , tuberculosis , bacteria , escherichia coli , medicine , pathology
Tuberculosis (TB), a disease caused by Mycobacterium tuberculosis, kills over 1 million people annually worldwide. With the development of multiple drug resistant strains of TB, interest has increased in the development of alternative methods for controlling this disease. As part of the HHMI‐SEA PHAGES project, bacteriophages capable of infecting Mycobacterium smegmatis, an organism in the same genus as M. tuberculosis, were isolated from soil samples in New Jersey. The phages were characterized by studying plaque morphology and by the use of Transmission Electron Microscopy. In addition, novel mycobacteriophages ShiVal and Alsfro were isolated and sequenced. The phage genomes were then annotated using DNA Master, BLAST, Glimmer, and GeneMark. Lastly, the information obtained was published in PhagesDB and GenBank. ShiVal is a B cluster, B1 subcluster phage with a genome size of 68K, 101 putative genes and 66.5% GC content. Alsfro is an A cluster, A1 subcluster phage with a genome size of 52K, 101 putative genes and a 63.3% GC content. Discovering and cataloging these novel phages is significant because a database of novel phages will be available to be tested for activity against M. tuberculosis. Grant Funding Source : HHMI SEA‐PHAGE