Premium
Three polymorphisms and susceptibility to breast cancer development: a Turkish study population (58.1)
Author(s) -
Bayram Banu,
Özkurt Mete,
Erkasap Nilüfer,
İhtiyar Enver,
Erkasap Serdar
Publication year - 2014
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.28.1_supplement.58.1
Subject(s) - turkish population , genotype , breast cancer , genetics , biology , allele , methylenetetrahydrofolate reductase , gene polymorphism , polymorphism (computer science) , gene , cancer
Objective: Genetic variations like polymorphisms underlie differences in our susceptibility to diseases. According to our literature reviews we have found three polymorphisms which contribute to breast cancer development from different pathways. However genetic variations of polymorphism vary between populations according to gene pools, life style and gene‐environment interactions. Based on these findings we aimed to investigate the association between p53 gene codon 72 polymorphism, MTHFR gene C677T polymorphism and PAI‐1 gene 4G/5G polymorphism and breast cancer in a Turkish study population. Material and Methods: Genomic DNA was extracted from 100 women (52 with breast cancer and 48 controls) in the study. PCR technique was used to amplify extracted DNA with proper primers for each polymorphism. For identifying genotypes PCR products were assessed with UV transilluminator by being exposed to agarose gel electrophoresis. Results: According to genotype distributions and allele frequencies of three polymorphisms there were no statistical differences between breast cancer patients and control group. Conclusion: As a conclusion of our study we may assert that p53 gene codon 72, MTHFR gene C677T and PAI‐1 gene 4G/5G polymorphisms should not be considered as susceptibility factors for breast cancer development. However polymorphism studies with larger numbers of the population provides more meaningful results. The significance of our result remains to be further investigated in different and even larger populations, combined with other polymorphisms considered as susceptibility factors for breast cancer.